R&D Systems™ Recombinant Human R-Spondin 2 Fc Chimera Protein, CF

Product Code. 18744484

Brand: R&D Systems™ 11427RS050

Description

Recombinant Protein

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells in the presence of Recombinant Human Wnt-3a (Catalog # 5036-WN ). The ED50 for this effect is 0.400-4.80 ng/mL.

Specifications

• Accession Number: Q6UXX9.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 340419
• Molecular Weight (g/mol): MolecularWeight-observed: 53-60 kDa, under reducing conditions., MolecularWeight-theroretical: 47 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 2 protein Human R-Spondin 2 (Ala32-Gly205) (N-terminus) Accession # Q6UXX9.2 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Cristin 2, CRISTIN2, hRspo2, MGC35555, MGC43342, Roof plate-specific spondin-2, RSPO2, RSpondin 2, R-spondin 2 homolog (Xenopus laevis), R-spondin-2
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: R-Spondin 2