R&D Systems™ Recombinant Human R-Spondin 1 Protein

Product Code.	Formulation	unitSize
18178530	Lyophilized from a 0.2 um filtered solution in PBS.	1mg
18137850	Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.	25µg
18106460	Lyophilized from a 0.2μm filtered solution in PBS.	25µg

Product Code. 18178530 Supplier R&D Systems™ Supplier No. 4645RS01M/CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

Order Info

Ships at ambient temperature.

Specifications

• Accession Number: Q2MKA7
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 um filtered solution in PBS.
• Gene ID (Entrez): 284654
• Molecular Weight (g/mol): 25.6 kDa
• Name: R-Spondin 1
• Quantity: 1 mg
• Source: Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein Ser21-Ala263
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 ug of the protein by the LAL method.
• Gene Symbol: RSPO1
• Conjugate: Unconjugated
• Cross Reactivity: R-Spondin 1
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 ug/mL in PBS.
• Purity or Quality Grade: 90%, by SDS-PAGE under reducing conditions and visualized by silver stain.