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Novus Biologicals™ Recombinant Human Protein Disulfide Isomerase/P4HB Protein
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Thiol Protein Reductase Activity is 0.001 change in 650nm/ min-2, determined by measuring the turbidity increase at 650 nm due to insulin reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase Activity is 0.5 umol active RNase A min-1 umol PDIA2, according to the re-activation of reduced and denatured RNase A.
Specifications
Specifications
| For Use With (Application) | PAGE, Bioactivity |
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0. |
| Gene ID (Entrez) | 5034 |
| Molecular Weight (g/mol) | M.W. (Theoretical): 56.6 kDa |
| Quantity | 1mg |
| Storage Requirements | Store at -20°C to -70°C as supplied. After reconstitution, store at 2°C to 8°C for 1 month and at -20°C to -70°C for long term storage. Avoid repeated freeze-thaw cycles. |
| Endotoxin Concentration | Less than 1 EU/μg of Protein Disulfide Isomerase/P4HB as determined by LAL method. |
| Gene Alias | Cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, DSI, ERBA2L, GIT, glutathione-insulin transhydrogenase, P4Hbeta, p55, PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1), PDIEC 5.3.4.1, PHDB, PO4DB, PO4HB, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide, PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase, thyroid hormone binding protein p55), Prolyl 4-hydroxylase subunit beta, prolyl 4-hydroxylase, beta polypeptide, protein disulfide isomerase family A, member 1, protein disulfide isomerase/oxidoreductase, protein disulfide isomerase-associated 1, protein disulfide-isomerase, protocollagen hydroxylase, thyroid hormone-binding protein p55 |
| Gene Symbol | P4HB |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20°C |
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