R&D Systems™ Recombinant Human PlGF Protein

Product Code.	Quantity	unitSize
18360288	10 μg	10µg
18198250	50μg	50µg

Product Code. 18360288 Supplier R&D Systems™ Supplier No. 264PGB010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 3516-FL ) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Human PlGF that produces 50% of the optimal binding response is approximately 0.75-4.5 ng/mL.

Specifications

• Accession Number: NP_001193941.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrier protein. Reconstitute (at 200 ug/mL in 4 mM HCl.)
• Gene ID (Entrez): 5228
• Molecular Weight (g/mol): Observed M.W.: 12-15 kDa, reducing conditions, Theoretical M.W.: 15 kDa
• Quantity: 10 μg
• Source: E. coli-derived human PlGF protein Ala21-Arg149
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 ug of the protein by the LAL method.
• Gene Alias: D12S1900, PGF, PGFL, placenta growth factor, placental growth factor, placental growth factor, vascular endothelial growth factor-related protein, PlGF, PlGF-2, PLGFplacental growth factor-like, SHGC-10760
• Common Name: PLGF
• Gene Symbol: PGF
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 Fc Chimera is immobilized (at 0.5 ug/mL, 100 uL/well, the concentration of Recombinant Human PlGF that produces 50% of the optimal binding response is approximately 0.15-0.9 ng/mL)
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Ala21-Arg149
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining