R&DSystems™ Recombinant Human PDGF-BB (CHO-expressed) Protein, CF

Product Code. 30058549

Brand: R&DSystems™ 11565PB050

Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 2.50-25.0 ng/mL.

Specifications

• Accession Number: P01127.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 5155
• Molecular Weight (g/mol): M.W.-observed: 11-13 kDa, M.W.-theroretical: 12 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human PDGF-BB protein Ser82-Thr190
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV, PDGFBB
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in 4mM HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PDGF-BB