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R&D Systems™ Recombinant Human PDGF-AB (single chain) Protein, CF

Product Code. 18754464
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18754464 10 μg 10µg
18794484 1 mg 1mg
18744464 50 μg 50µg
18724354 100 μg 100µg
18784484 250 μg 250µg
18744564 500 μg 500µg
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Product Code. 18754464 Supplier R&D Systems™ Supplier No. 11471AB010

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Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 2.00-20.0 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_001382295.1 & P01127.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Gene ID (Entrez) 5154
Molecular Weight (g/mol) MolecularWeight-observed: 28-37 kDa, under reducing conditions., MolecularWeight-theroretical: 26 kDa
Quantity 10 μg
Source Human embryonic kidney cell, HEK293-derived human PDGF-AB protein Human PDGFA (Ser87-Arg196) (Cys123Ala, Cys132Ala) (N-terminus) Accession # NP_001382295.1 linker Human PDGFB (Ser82-Thr190) (Cys123Ala, Cys132Ala) (N-terminus) Accession # P01127.1 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Reconstitution Reconstitute 10 μg size at 100 μg/mL in 4 mM HCl. Reconstitute all the other sizes at 250 μg/mL in 4 mM HCl.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PDGF-AB
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