R&D Systems™ Recombinant Human MSP/MST1 (Cys672Ala) Protein

Product Code. 18107900

Brand: R&D Systems™ 4306MS010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the recombinant human MSP (C672A) significantly induces phosphorylation of MSP R/Ron measured by the Human Phospho-MSP R/Ron DuoSet™ IC Kit (Catalog # DYC1947 ). Measured by its binding ability in a functional ELISA. When Recombinant Human MSPR/Ron Protein (Catalog #1947-MS) is represent at 100 ng/mL (100 μg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 4-32 ng/mL.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 4485
• Molecular Weight (g/mol): 53 kDa ( alpha-chain), 25 kDa (beta-chain)
• Name: MSP/MST1 (Cys672Ala)
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human MSP/MST1 protein Gln19-Arg483 (alpha) & Val484-Gly711 (Cys672Ala) (beta)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked heterodimer
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.