Novus Biologicals™ Recombinant Human ME2 His (C-Term) Protein

Product Code.	Quantity	unitSize
18421172	500 μg	500µg
18497251	100 μg	100µg
18494212	5 μg	5µg
18451562	1 mg	1mg

Product Code. 18421172 Supplier Novus Biologicals™ Supplier No. NBP234980500ug

Description

Recombinant Protein

Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 +/- 0.6 mM.

Specifications

• Accession Number: P23368
• Concentration: LYOPH
• For Use With (Application): PAGE, Bioactivity
• Formulation: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Gene ID (Entrez): 4200
• Molecular Weight (g/mol): 64.4 kDa
• Quantity: 500 μg
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2°C to 8°C for 1 month and at -20°C to -70°C for long term storage. Avoid repeated freeze-thaw cycles.
• Endotoxin Concentration: Less than 1 EU/ug of ME2 as determined by LAL method.
• Gene Alias: EC 1.1.1, EC 1.1.1.38, malate dehydrogenase, Malic enzyme 2, malic enzyme 2, NAD(+)-dependent, mitochondrial, NAD-dependent malic enzyme, mitochondrial, NAD-ME, ODS1, pyruvic-malic carboxylase
• Gene Symbol: ME2
• Reconstitution: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20°C.
• Research Category: Biologically Active Proteins
• Purity or Quality Grade: >95% by SDS-PAGE and HPLC
• Protein: ME2