R&D Systems™ Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein

Product Code.	Quantity	unitSize
18379437	50 μg	50µg

Product Code. 18379437 Supplier R&D Systems™ Supplier No. 2078T4050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN ) is coated at 4 μg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera binds with a typical ED50 of 0.15-0.9 μg/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera immobilized at 35 μg/mL, 100 μL/well, will mediate >40% HSB-2 cell adhesion (105 cells/well).

Specifications

• Accession Number: Q8N423
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 10288
• Molecular Weight (g/mol): M.W. (Observed): 94-100 kDa, reducing conditions; M.W. (theoretical): 73.9 kDa (monomer)
• Name: ILT4/CD85d Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human LILRB2/CD85d/ILT4 protein Human LILRB2/CD85d/ILT4 (Gly24-His458) Accession # (N-terminus)AAH36827.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD85 antigen-like family member D, CD85d, CD85d antigen, Ig-like transcript 4, ILT4, ILT-4, ILT4CD85d, Immunoglobulin-like transcript 4, Leukocyte immunoglobulin-like receptor 2, leukocyte immunoglobulin-like receptor subfamily B member 2, leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), LIR2, LIR2CD85D, LIR-2 subfamily A (with TM domain), member 6, member 2, MIR10, MIR10LILRA6, Monocyte/macrophage immunoglobulin-like receptor 10
• Gene Symbol: LILRB2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: ILT4/CD85d/LILRB2