Novus Biologicals™ Recombinant Human LIF Protein

Product Code. 18412472

Brand: Novus Biologicals™ NBP2349351mg

Description

Recombinant Protein

LIF Protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of >1.0 x 10⁷ IU/mg.

Specifications

• Accession Number: P15018, P15018, P15018
• Concentration: LYOPH
• For Use With (Application): PAGE, Bioactivity
• Formulation: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Gene ID (Entrez): 3976
• Molecular Weight (g/mol): 19.7 kDa
• Quantity: 1 mg
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2°C to 8°C for 1 month and at -20°C to -70°C for long term storage. Avoid repeated freeze-thaw cycles.
• Endotoxin Concentration: Less than 1 EU/ug of LIF as determined by LAL method.
• Gene Alias: CDF, D factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDAcholinergic differentiation factor, leukemia inhibitory factor, leukemia inhibitory factor (cholinergic differentiation factor), Melanoma-derived LPL inhibitor, MLPLI
• Gene Symbol: LIF
• Reconstitution: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.2mg/mL. Apportion stock solutions into working aliquots and store at <-20°C.
• Research Category: Biologically Active Proteins, Cell Cycle and Replication, Stem Cell Lines
• Purity or Quality Grade: >98% pure by SDS-PAGE and HPLC
• Protein: LIF