R&D Systems™ Recombinant Human IL-6, Animal-Free Protein

Product Code.	Quantity	unitSize
30713259	20 μg	20µg

Product Code. 30713259 Supplier R&D Systems™ Supplier No. AFL206020

Description

Intended for preclinical researchers who may transition to GMP IL-6 for their clinical work

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.200-0.800 ng/mL. The specific activity of Recombinant Human IL-6 is >1.00 x 108 IU/mg, which is calibrated against the human IL-6 WHO International Standard (NIBSC code: 89/548).

Specifications

• Accession Number: P05231.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Gene ID (Entrez): 3569
• Molecular Weight (g/mol): M.W.-theroretical: 20.9 kDa
• Quantity: 20 μg
• Source: E. coli-derived human IL-6 protein Pro29-Met212 Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
• Gene Symbol: IL6
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Purity or Quality Grade: Antigen Affinity-purified
• Protein: IL-6