R&D Systems™ Recombinant Human IL-35 Fc Chimera Protein

Product Code. 18785547

Brand: R&D Systems™ 8608IL050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human IL-35 Fc Chimera Protein is derived from HEK293. The Recombinant Human IL-35 Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 10148
• Molecular Weight (g/mol): Observed M.W.: 81-97kDa, reducing conditions, Theoretical M.W.: 73kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell,HEK293-derived human IL-35 protein Human IL-27 beta/EBI3 (Arg21-Lys229) Accession &Num; Q14213 (GGGS)4 Human IL-12 alpha/p35 Arg23-Ser219 Accession &Num; P29459 IEGRMD Human IgG1 Pro100-Lys330)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: IL-35
• Gene Symbol: EBI3
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 R beta 2 Fc Chimera is immobilized at 5μg/mL (100μL/well), the concentration of Recombinant Human IL-35 Fc Chimera that produces 50% optimal binding response is approximately 20-120ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Human IL-27 beta/EBI3 (Arg21-Lys229) Accession # Q14213, N-terminus; (GGGS)4; Human IL-12 alpha/p35 Arg23-Ser219 Accession # P29459; IEGRMD; Human IgG1 Pro100-Lys330), C-terminus
• Purity or Quality Grade: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain