R&D Systems™ Recombinant Human IL-2 (C145S, HEK293-expressed) Protein

Product Code. 18777636

Brand: R&D Systems™ 11218IL010

Description

Contains the Cysteine to Serine substitution found in Proleukin™ (aldesleukin)

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL.

Specifications

• Accession Number: P60568.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 3558
• Molecular Weight (g/mol): M.W.-observed: 14-18 kDa, under reducing conditions., M.W.-theroretical: 15 kDa
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-2 protein Ala21-Thr153 (Cys145Ser)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: aldesleukin, IL2, IL-2, IL-2 lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, Proleukin, T cell growth factor, T-cell growth factor, TCGF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-500 μg/mL in sterile 100 mM Acetic Acid.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-2