R&D Systems™ Recombinant Human IL-18 R alpha/IL-1 R5 Avi His Protein

Product Code. 18796086

Brand: R&D Systems™ AVI11179050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R beta/IL-1 R7 Fc (Catalog # 118-AP ) is immobilized at 0.500 μg/mL (100 μL/well), in the present of Recombinant Human IL-18 (Catalog # 9124-IL ) Biotinylated Recombinant Human IL-18 R alpha/IL-1 R5 Avi-tag His-tag (Catalog # AVI11179) binds with an ED50 of 20.0-200 ng/mL. The biotin to protein ratio is great than 0.7 as determined by the HABA assay.

Specifications

• Accession Number: Q13478.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W.-observed: 63-71 kDa, under reducing conditions., M.W.-theroretical: 40 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-18 R alpha/IL-1 R5 protein Human IL-18 R alpha/IL-1 R5 (Cys22-Arg329) (C-terminus) Accession # Q13478.1 Avi-tag 6-His tag (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.