R&D Systems™ Recombinant Human IL-18/IL-1F4 GMP Protein, CF

Product Code. 30713257

Brand: R&D Systems™ BT018GMP050

Description

Recombinant Protein

Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha . The ED50 for this effect is 1.25-15.0 ng/mL. The specific activity of GMP Recombinant Human IL-18/IL-1F4 is > 5.00 x 106 units/mg, which is calibrated against the human IL-18 WHO Standard (NIBSC code: 03/200).

Specifications

• Accession Number: Q14116.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS and TCEP with Trehalose.
• Gene ID (Entrez): 3606
• Molecular Weight (g/mol): M.W.-observed: 18 kDa, under reducing conditions., M.W.-theroretical: 18 kDa
• Quantity: 50 μg
• Source: E. coli-derived human IL-18/IL-1F4 protein Tyr37-Asp193 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidlines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Iboctadekin, IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL18, IL-18MGC12320, IL-1F4, IL1F4iboctadekin, IL-1g, Interferon gamma-inducing factor, interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, interleukin-18
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in sterile water.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 18205 Da ± 5 Da
• Protein: IL-18/IL-1F4