R&D Systems™ Recombinant Human gp130 His-tag Avi-tag Protein

Product Code.	Quantity	unitSize
18749826	50 μg	50µg

Product Code. 18749826 Supplier R&D Systems™ Supplier No. AVI11067050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human gp130 His-tag Avi-tag is captured on a Streptavidin Coated Plate (Catalog # CP004 ), it binds to Recombinant Human IL-6 (Catalog # 7270-IL ) in the presence of Recombinant Human IL-6R alpha (Catalog # 227-SR ). The ED50 for this binding is 0.800-4.80 ng/mL.

Specifications

• Accession Number: AAA59155.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3572
• Molecular Weight (g/mol): MolecularWeight-observed: 93-103 kDa, under reducing conditions.; MolecularWeight-theroretical: 70 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human gp130 protein Human gp130 (Glu23-Ile618) (N-terminus) Accession # AAA59155.1 6-His tag Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD130, CD130 antigen, CDw130, DKFZp564F053, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, IL-6 receptor subunit beta, IL-6 R subunit beta, IL-6 RB, IL-6 R-beta, IL6ST, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, Interleukin-6 signal transducer, Membrane glycoprotein 130, membrane glycoprotein gp130, Oncostatin-M receptor subunit alpha
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: gp130/CD130