R&D Systems™ Recombinant Human Flt-3 Ligand/FLT3L Protein, Animal-Free

Product Code. 18735775

Brand: R&D Systems™ BTFT3LAFL050

Description

Intended for preclinical researchers who may transition to GMP Flt-3 Ligand/FLT3L for their clinical work

Measured in a cell proliferation assay using OCI-AML5 acute myeloid leukemia cells. The ED50 for this effect is 0.200-2.00 ng/mL. The specific activity of Recombinant Human Flt-3 Ligand/FLT3L is >5.00 x 105 units/mg, which is calibrated against the human Flt-3 Ligand WHO Standard (NIBSC code: 96/532).

Specifications

• Accession Number: P49771.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 2323
• Molecular Weight (g/mol): M.W. (Observed): 17 kDa, under reducing conditions., M.W. (Theroretical): 18 kDa
• Quantity: 50 μg
• Source: E. coli-derived human Flt-3 Ligand/FLT3L protein Thr27 - Ala181 Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: FL, Flt3 ligand, FLT3L, fms-related tyrosine kinase 3 ligand, SL cytokine
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Flt-3 Ligand/FLT3L