R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein

Product Code. 18716856

Brand: R&D Systems™ 233FB010/CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.

Specifications

• Accession Number: P09038
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 16.5 kDa
• Quantity: 10 μg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Pro143-Ser288,with an N-terminal Ala
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Gene Symbol: FGF2
• Conjugate: Unconjugated
• Cross Reactivity: FGF basic/FGF2
• Species: Human
• Reconstitution: Reconstitute at 100-250 μg/mL in sterile PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.