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R&D Systems™ Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein
Description
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (Catalog # AVI10468) with an ED50 of 4-40 ng/mL.
Specifications
Specifications
| Accession Number | AAH17865.1 |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Gene ID (Entrez) | 2214 |
| Molecular Weight (g/mol) | Observed: 48-57 kDa, under reducing conditions; Theroretical: 24 kDa |
| Quantity | 50 μg |
| Source | Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein Human Fc gamma RIIIA/CD16a (Gly17-Gln208) Accession # AAH17865.1(N-terminus) 6-His tag Avi-tag N-terminus C-terminus |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.10 EU / 1 μg of the protein by the LAL method. |
| Conjugate | Biotin |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
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