R&D Systems™ Recombinant Human DLL4 Fc Chimera Protein

Product Code. 18766976

Brand: R&D Systems™ 10185D4050

Description

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands

• Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis
• Dysregulation in the Notch pathway is associated with various human diseases
• Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ fort his effect is 0.02-0.12 μg/mL.

Specifications

• Accession Number: Q9NR61
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 54567
• Molecular Weight (g/mol): 81 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 50 μg
• Source:

  Human embryonic kidney cell, HEK293-derived human DLL4 protein

  Human DLL4 (Ser27-Pro524) Accession # Q9NR61IEGRMDPHuman IgG1 (Pro100-Lys330)N-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 250 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS