R&D Systems™ Recombinant Human Dkk-1 Protein, CF

Product Code. 18796165

Brand: R&D Systems™ 11387DK01M

Description

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 8.00-96.0 ng/mL.

Specifications

• Accession Number: O94907.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 22943
• Molecular Weight (g/mol): M.W. (Observed): 38-42 kDa, under reducing conditions., M.W. (Theroretical): 26 kDa
• Quantity: 1 mg
• Source: Human embryonic kidney cell, HEK293-derived human Dkk-1 protein Thr32-His266
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, DKK-1, hDkk-1, SKdickkopf-1 like
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Dkk-1