R&D Systems™ Recombinant Human Activin A (CHO derived) Protein

Product Code. 18746335

Brand: R&D Systems™ 11348AC01M

Description

Carrier Free Recombinant Protein

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.200-1.60 ng/mL.

Specifications

• Accession Number: P08476.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 3624
• Molecular Weight (g/mol): MolecularWeight-observed: 13 kDa under reducing conditions and 24 kDa under non-reducing conditions., MolecularWeight-theroretical: 13 kDa
• Quantity: 1 mg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Activin A protein Gly311-Ser426
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: activin AB alpha polypeptide, Activin beta-A chain, erythroid differentiation factor, Erythroid differentiation protein, follicle-stimulating hormone-releasing protein, FSH-releasing protein, inhibin beta A chain, inhibin beta A subunit, Inhibin, beta-1
• Conjugate: Unconjugated
• Reconstitution: Reconstitute 20 μg size at 100 μg/mL in sterile 4 mM HCl. Reconstitute all the other sizes (at 500 μg/mL in sterile 4 mM HCl.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Activin A