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R&D Systems™ Recombinant Human Active Heparanase/HPSE Protein
Description
Measured by its ability to release biotinylated heparan sulfate from Recombinant Human Syndecan-4 (Catalog # 2918-SD ). 20 ng of Recombinant Human Active Heparanase/HPSE digestion will result in >50% of OD reduction compared with the Negative Control, as measured under the described conditions.
Specifications
Specifications
| Accession Number | AF144325 |
| For Use With (Application) | Enzyme Activity |
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and E64. |
| Gene ID (Entrez) | 10855 |
| Molecular Weight (g/mol) | M.W. (Observed): 50-60 kDa & 7-9 kDa, reducing conditions; M.W. (theoretical): 43 kDa & 9 kDa |
| Name | Active Heparanase/HPSE |
| Quantity | 5 μg |
| Source | Chinese Hamster Ovary cell line, CHO-derived human Heparanase/HPSE protein Gln36-Ile543 with N-terminal 6-His tag The linker peptide from Ser110-Gln157 is removed by protease treatment |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. |
| Endotoxin Concentration | <1.0 EU / 1 μg of the protein by the LAL method. |
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