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Novus Biologicals™ Recombinant Bacteria Flagellin NLRC4 Mutant His Protein

Product Code. 18362402
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Quantity:
10 μg
Unit Size:
10µg
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Product Code. Quantity unitSize
18362402 10 μg 10µg
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Product Code. 18362402 Supplier Novus Biologicals™ Supplier No. NBP318158

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Description

Recombinant Protein

Detected by human & mouse TLR5. Not detected by mouse NLRC4. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at 5-50ng/mL & in vivo at a starting concentration of 0.1-1 ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages (in the presence of streptolysin O (SLO)). It can be used as negative control for NLRC4 signaling.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_460912
Concentration LYOPH
For Use With (Application) Bioactivity
Formulation Lyophilized from PBS
Quantity 10 μg
Storage Requirements Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles.
Endotoxin Concentration <0.01 EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)
Research Category Immunology, Innate Immunity
Purity or Quality Grade >95% pure by SDS-PAGE
Protein Flagellin
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