Proteintech™ Rat Total MMP-9 ELISA Kit

Product Code. 16871646

Brand: Proteintech™ KE2000696T

Description

KE20006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Rat total MMP9 ELISA kit is to be used to detect and quantify protein levels of endogenous rat total MMP9.

• The assay recognizes rat total MMP9. An antibody specific for rat total MMP9 has been pre-coated onto the microwells.
• The rat total MMP9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat total MMP9 is added to detect the captured rat total MMP9 protein.
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.MMP9 (matrix metallopeptidase 9), also named as Gelatinase B, is a member of matrix metalloproteinase (MMP) family.
• The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis, tissue remodelling and many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration.
• MMP9 is produced by a variety of normal and transformed cells including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts and so on.
• Transgenic mouse models report that MMP9 contributes to skin carcinogenesis, suppresses development of experimental abdominal aortic aneurysms, and triggers the angiogenic switch during carcinogenesis.

Specifications

• Accession Number: NM_031055
• Assay Range: 62.5 to 4000 pg/mL
• Assay Sensitivity: 14.9 pg/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Rat
• Gene ID (Entrez): 81687
• Host Species: Rat
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 4.5 hr.
• UniProt ID: P50282

Safety and Handling

Product Identifier

• 96TEST Rat Total MMP-9 ELISA Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine