Invitrogen™ Rat IL-4 ELISA Kit

Product Code. 15522067

Brand: Invitrogen™ BMS628TEN

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-4
Biotin-Conjugate anti-rat IL-4 monoclonal antibody
Streptavidin-HRP
Rat IL-4 Standard lyophilized, 200pg/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Rat Interleukin 4 (Rt IL-4) ELISA quantitates Rt IL-4 in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat IL-4. Principle of the method The Rat IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Accession Number: P20096
• Assay Range: 1.6 to 100 pg/mL
• Assay Sensitivity: 0.2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Target Species: Rat
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 287287
• Gene Symbol: Il4e12
• Interassay CV: 10%
• Intraassay CV: 5
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: B-cell stimulatory factor 1, B cell growth factor 1
• Target: IL-4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 10 mins