Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Isotype Control

Product Code	Brand	Quantity
15380950	Affymetrix eBioscience 25-4031-81	50 μg
15390950	Affymetrix eBioscience 25-4031-82	100 μg

Description

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: PE-Cy7 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.

Specifications

• Antigen: Rat IgG2b kappa
• Concentration: 0.2 mg/mL
• Conjugate: PE-Cyanine7
• Host Species: Rat
• Target Species: Not Applicable
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: eB149/10H5
• Applications: Control, Flow Cytometry
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography

For Research Use Only.