Invitrogen™ Fractalkine-Cell Lysates (CX3CL1) Rat ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ ERCXCL1CL

Product Code. 15445373

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Rat Fractalkine-Cell Lysate (CX3CL1) ELISA quantitates Rt Fractalkine in rat cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Rt Fractalkine. Principle of the method The Rat Fractalkine solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

Specifications

• Accession Number: O55145
• Assay Sensitivity: 5 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Cell Lysates
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 89808
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Oncology
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 2.74-2000 pg/mL
• Calibration Range: 5pg/mL to 2000pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Specificity: The antibody pair provided in this kit recognizes rat Fractalkine.
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Cx3c,Scyd1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Cell Lysate,20 μL
• Synonym: Cx3c, Scyd1
• Test Time: 1 hr. 20 min.