Proteintech™ Rat FABP1 ELISA Kit

Product Code. 16821656

Brand: Proteintech™ KE2001196T

Description

KE20011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP1 ELISA kit is to be used to detect and quantify protein levels of endogenous FABP1.

• The assay recognizes rat FABP1. An antibody specific for FABP1 has been pre-coated onto the microwells. The FABP1 protein in samples is captured by the coated antibody after incubation.
• Following extensive washing, another antibody of biotinylated specific for rat FABP1 is added to detect the captured rat FABP1 protein.
• For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
• Fatty acid‐binding protein 1 (FABP1, also known as L‐FABP) is a 14 KDa cytosolic protein abundantly expressed in the liver.
• It can bind fatty acids and many other molecules and is involved in the uptake, transport, and metabolism of cellular long-chain fatty acids and other lipid ligands. Higher FABP1 serum level has been associated with liver injury.
• Levels of FABP1 can be helpful as a biomarker for metabolic disturbances and impaired function of hepatocytes.

Specifications

• Accession Number: BC086947
• Assay Range: 78.1 to 5000 pg/mL
• Assay Sensitivity: 8.1 pg/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Rat
• Gene ID (Entrez): 24360
• Host Species: Rat
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 4.5 hr.
• UniProt ID: P02692

Safety and Handling

Product Identifier

• 96TEST Human Adiponectin ELISA Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine