CD16/CD32 Rat anti-Mouse, Brilliant Violet 510, Clone: 2.4G2, BD Optibuild™

Rat Monoclonal Antibody

Brand:  BD Biosciences 740111

Product Code. 15876489

Description

The 2.4G2 antibody specifically recognizes a common nonpolymorphic epitope on the extracellular domains of the mouse Fc γIII (CD16) and Fc γII (CD32) Receptors. It has also been reported to bind the Fc γI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the Fc γIII and Fc γII, and possibly Fc γI, Receptors . CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD16/CD32
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: FcγRIII/FcγRII; Fcgr3/Fcgr2
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: 2.4G2
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse BALB/c Macrophage J774
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only