CD45 Rat anti-Mouse, APC-R700, Clone: 30-F11, BD

Rat Monoclonal Antibody

Brand:  BD Biosciences 565478

Product Code. 15804349

Description

The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

Specifications

• Antigen: CD45
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Ptprc; CD45R; LCA; Leukocyte common antigen; T200; B220; Ly-5; Lyt-4
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: 30-F11
• Conjugate: APC-R700
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Mouse Thymus/Spleen
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.