Invitrogen™ Rat Agrin ELISA Kit

Product Code. 17145043

Brand: Invitrogen™ ERA2RB

Description

ELISA

Rat Agrin quantitates rat Agrin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat Agrin.

Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designatedy andz display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.

Specifications

• Accession Number: AGR; AGRIN; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 22 kDa fragment; Agrin C-terminal 90 kDa fragment; Agrin N-terminal 110 kDa subunit; agrin proteoglycan; AGRN; C22; C90; CMS8; CMSPPD; nmf380; RP23-139J18.2
• Assay Range: 0.06 to 50 ng/mL
• Assay Sensitivity: 0.06 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 25592
• Gene Symbol: Agrn
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Agrin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins