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Thermo Scientific™ RapidFinder™ Basa ID Kit
Description
The Thermo Scientific™ RapidFinder™ Basa ID Kit enables the simple and reliable detection of basa (Pangasius hypophthalmus) DNA in food and feed samples.
The RapidFinder™ Basa ID Kit enables the simple and reliable detection of Basa DNA in food and feed samples.
The RapidFinder™ Basa ID Kit enables the simple and reliable detection of Basa DNA in food and feed samples. The method works by detection of basa (Pangasius hypophthalmus) DNA that is present in food and feed samples by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Basa ID Kit detects and provides sensitivity down to 0.01% of basa (Pangasius hypophthalmus) DNA. The included positive control in the kit, which comprises 0.1% Basa DNA, can be used as a reference to set the threshold of detection of the assay.
- Simple- Real-time PCR based assay, no electrophoresis needed
- Flexible- Basa DNA detected in any food or feed sample, both raw and processed
- Reliable- Internal Positive Control (IPC) provides process control check for PCR inhibitors
- Fast- From sample to result in the same day
The assay is compatible with Applied Biosystems™ QuantStudio 5™ and 7500 Fast Real-Time PCR Systems.
Sample Preparation
DNA sample preparation is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using up to 20 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.
Part of a Complete Portfolio of Solutions for Fish Species Detection
The RapidFinder™ Basa ID Kit is just one of our many available kits for the sensitive detection of various fish species in food and feed samples. Other RapidFinder™ Fish species identification kits available include those for the detection of Gadus, Pollack, Pollock, Haddock, Tuna, and Specific Tuna.
Specifications
Specifications
| Content And Storage | Contains 1 tube each of: Basa Master Mix (store at -20°C) General Master Mix (store at -20°C upon receipt; 2-8 °C after initial use) Positive Control (store at -20°C) |
| Description | Basa ID Kit |
| Detection Method | Primer-probe |
| PCR Method | qPCR |
| Reaction Speed | Standard |
| For Use With (Equipment) | QuantStudio 5™ and 7500 Fast |
| Product Line | RapidFinder™ |
| Product Type | RapidFinder™ |
| Quantity | 48 reactions |
| Sensitivity | 0.01% of Basa (Pangasius hypophthalmus) DNA |
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The RapidFinder™ Species Identification Kits are made available through a partnership with Health in Code, S.L.
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