Invitrogen™ RAPGEF2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5139893

Product Code. 17844410

Beskrivelse

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. Immunogen sequence: PPPAHKINQG LQVPAVSLYP SRKKVPVKDL PPFGINSPQA LKKILSLSEE GSLERHKKQA EDTISNASSQ LSSPPTSP Highest antigen sequence indentity to the following orthologs: Rat - 94%, Mouse - 94%.

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP- induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain- derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

Tekniske data

• Antigen: RAPGEF2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RAPGEF2
• Gene Alias: 5830453M24Rik; CNrasGEF; Cyclic nucleotide ras GEF; fa11h07; Kiaa0313; mKIAA0313; Neural RAP guanine nucleotide exchange protein; nRap GEP; NRAPGEP; PDZ domain containing guanine nucleotide exchange factor (GEF) 1; PDZ domain-containing guanine nucleotide exchange factor 1; PDZGEF1; PDZ-GEF1; RA(Ras/Rap1A-associating)-GEF; RA-GEF; RA-GEF-1; Rap guanine nucleotide exchange factor (GEF) 2; Rap guanine nucleotide exchange factor 2; RAPGEF2; Rap-GEP; Ras/Rap1-associating GEF-1; wu:fa11h07
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9693
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9Y4G8
• Gene Symbols: RAPGEF2
• Immunogen: Recombinant protein corresponding to Human RAPGEF2. Recombinant protein control fragment (Product #RP-109168).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG