MAP3K5 (phospho S966), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP3K5.

Brand:  Abnova PAB16964.100ug

Product Code. 16072507

Description

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq

Specifications

• Antigen: MAP3K5
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP3K5.
• Formulation: In 20mM PBS, 0.15M NaCl, pH 7.2 (0.01% sodium azide)
• Gene Alias: ASK1/MAPKKK5/MEKK5
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody detects endogenous levels of MAP3K5 only when phosphorylated at serine 966.
• Content And Storage: Store at 4°C for three months. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

• Applications: ELISA, Immunohistochemistry (PFA fixed), Western Blot
• Conjugate: Unconjugated
• Dilution: Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) ELISA (1:4000) The optimal working dilution should be determined by the end user.
• Gene: MAP3K5
• Gene Symbols: MAP3K5
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S966 of human MAP3K5.
• Regulatory Status: RUO
• Gene ID (Entrez): 4217
• Target Species: Human, Mouse, Rat
• Form: Liquid

For Research Use Only