AKT1 (phospho T34), Rabbit, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.

Brand:  Abnova PAB7912.100uL

Product Code. 16080728

Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq

Specifications

• Antigen: AKT1
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
• Formulation: In PBS (50% glycerol, 1mg/mL BSA, 0.05% sodium azide)
• Gene Alias: AKT/MGC99656/PKB/PKB-ALPHA/PRKBA/RAC/RAC-ALPHA
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Test Specificity: This sequence is highly conserved in rat and mouse Akt.
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Dilution: ELISA (1:2000) Western Blot (1:500) The optimal working dilution should be determined by the end user.
• Gene: AKT1
• Gene Symbols: AKT1
• Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T34 of human AKT1.
• Regulatory Status: RUO
• Gene ID (Entrez): 207
• Target Species: Human, Rabbit, Rat
• Form: Liquid

For Research Use Only