ABL1 (phospho Y412), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.

Brand:  Abnova PAB16947.100ug

Product Code. 16012497

Description

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq

Specifications

• Antigen: ABL1
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.
• Formulation: In 20mM PBS, 0.15M NaCl, pH 7.2 (0.01% sodium azide)
• Gene Accession No.: P00519
• Gene Symbols: ABL1
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Y412 of human ABL1.
• Regulatory Status: RUO
• Gene ID (Entrez): 25
• Target Species: Human, Mouse, Rat
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (PFA fixed), Western Blot
• Conjugate: Unconjugated
• Dilution: Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) ELISA (1:4000) The optimal working dilution should be determined by the end user.
• Gene: ABL1
• Gene Alias: ABL/JTK7/bcr/abl/c-ABL/p150/v-abl
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody detects endogenous levels of ABL1 only when phosphorylated at tyrosine 412.
• Content And Storage: Store at 4°C for three months. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

For Research Use Only