Invitrogen™ RAB9 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA595339

Product Code. 16364605

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human HepG2 whole cell, human 293T whole cell, monkey COS-7 whole cell, mouse NIH/3T3 whole cell. ICC/IF: Hela cell. Flow: U20S cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Specifications

• Antigen: RAB9
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rab9a
• Gene Alias: 2410064E05Rik; AI195561; rab9; rab9 GTP-binding protein; RAB9, member RAS oncogene family; Rab9a; rab-9A; RAB9A, member RAS oncogene family; RAB9-like protein; ras-related protein Rab-9A; Sid 99; Sid6061p; Sid99
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56382, 9367
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene Accession No.: P51151, Q9R0M6
• Gene Symbols: Rab9, Rab9a
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9 (156-193aa KDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRK).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Monkey
• Product Type: Antibody
• Isotype: IgG