R&D Systems™ Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Product Code. 18771578

Brand: R&D Systems™ 211TBB010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.

Order Info

The product is shipped at ambient temperature. Upon receipt, store it immediately

Specifications

• Accession Number: P01374
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 4049
• Molecular Weight (g/mol): M.W. (Observed): 17-19 kDa, reducing conditions; M.W. (theoretical): 19 kDa
• Name: Lymphotoxin-alpha/TNF-beta
• Preparation Method: Reconstitute at 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• Quantity: 10 μg
• Source: E. coli-derived human Lymphotoxin-alpha/TNF-beta protein Leu35-Leu205, with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: LTA
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Form: Powder
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Lymphotoxin-alpha/TNF-beta