R&D Systems™ Recombinant Human VEGF 165b Protein

Product Code.	Formulation	unitSize
18118080	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.	25µg
18360298	Lyophilized from a 0.2μm filtered solution in PBS.	25µg

Product Code. 18118080 Supplier R&D Systems™ Supplier No. 3045VE025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. Serially diluted Recombinant Human (rh) VEGF165b is added to a Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD ) coated plate (2 μg/mL, 100 μL/well). Bound rhVEGF165b is detected by a human VEGF165b specific antibody (Catalog # MAB3045 ). The concentration of rhVEGF165b that produces half-maximum response is approximately 0.12-0.6 nM.

Specifications

• Accession Number: AAL27435
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): M.W. (Observed): 19-24 kDa, reducing conditions; M.W. (theoretical): 19.1 kDa (monomer)
• Name: VEGF 165b
• Quantity: 25 μg
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived human VEGF protein Ala27-Asp191
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: VEGFA
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: VEGF