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R&D Systems™ Recombinant Human IL-2 Protein
Description
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Specifications
Specifications
| Accession Number | P60568 |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Gene ID (Entrez) | 3558 |
| Molecular Weight (g/mol) | 13 kDa, under reducing conditions. |
| Name | IL-2 |
| Quantity | 10 μg |
| Source | E. coli-derived human IL-2 protein Ala21-Thr153, with an N-terminal Met |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.10 EU / 1 μg of the protein by the LAL method. |
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