R&D Systems™ Recombinant Human FGF-4 (aa 71-206) Protein

Product Code. 18108670

Brand: R&D Systems™ 7460F4025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.25-1.25 ng/mL.

Specifications

• Accession Number: P08620
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
• Gene ID (Entrez): 2249
• Molecular Weight (g/mol): M.W. (Observed): 14 kDa, reducing conditions; M.W. (theoretical): 15.2 kDa
• Name: FGF-4 (aa 71-206)
• Quantity: 25 μg
• Source: E. coli-derived human FGF-4 protein Ser71-Leu206 with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Monomer
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: FGF4
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: FGF-4