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R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein
A New rh CD40 Ligand is Available! ∼1000 fold better activity; HEK293 expressed; HA tag!
POA
Specifications
| Accession Number | P29965 |
|---|---|
| For Use With (Application) | Bioactivity |
| Gene ID (Entrez) | 959 |
| Molecular Weight (g/mol) | M.W. (Observed): 18 kDa, reducing conditions; M.W. (theoretical): 16.9 kDa (monomer) |
| Name | CD40 Ligand/TNFSF5 aa 108-261 |
Description
Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 1-3.00 μg/mL in the presence of 20 ng/mL of recombinant human IL-4.Specifications
| P29965 | |
| 959 | |
| CD40 Ligand/TNFSF5 aa 108-261 | |
| E. coli-derived human CD40 Ligand/TNFSF5 protein Glu108-Leu261, with an N-terminal Met | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Bioactivity | |
| M.W. (Observed): 18 kDa, reducing conditions; M.W. (theoretical): 16.9 kDa (monomer) | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 3 months, 2°C to 8°C under sterile conditions after reconstitution. | |
| CD40LG | |
| Recombinant | |
| CD40 Ligand/TNFSF5 |