R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) Protein

Product Code. 18167060

Brand: R&D Systems™ 6245CL050

Description

A New rh CD40 Ligand is Available! ∼1000 fold better activity; HEK293 expressed; HA tag!

Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 1-3.00 μg/mL in the presence of 20 ng/mL of recombinant human IL-4.

Specifications

• Accession Number: P29965
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
• Gene ID (Entrez): 959
• Molecular Weight (g/mol): M.W. (Observed): 18 kDa, reducing conditions; M.W. (theoretical): 16.9 kDa (monomer)
• Name: CD40 Ligand/TNFSF5 aa 108-261
• Quantity: 50 μg
• Source: E. coli-derived human CD40 Ligand/TNFSF5 protein Glu108-Leu261, with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 3 months, 2°C to 8°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD40LG
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: CD40 Ligand/TNFSF5