Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits

Product Code.	Quantity	Assay	Quantitation Range	unitSize
15850210	100 assays	1X dsDNA, high sensitivity	0.1 to 120 ng	Each
15860210	500 assays	1X dsDNA, high sensitivity	0.1 to 120 ng	Each
17104589	500 assays	1X dsDNA, broad range	4 to 4000 ng	Pack of 500
17194104	100 assays	1X dsDNA, broad range	4 to 4000 ng	67g

Product Code. 15850210 Supplier Invitrogen™ Supplier No. Q33230

Description

Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.

Experience easy, accurate, and precise quantification of dsDNA with Qubit 1X dsDNA HS (High Sensitivity) and Qubit 1X dsDNA BR (Broad Range) Assay Kits. The 1X formulation provides a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometers. These assays are highly selective for dsDNA over ssDNA, RNA, protein, and free nucleotides. Contaminants, such as salts, solvents, or detergents are well-tolerated.

Qubit 1X dsDNA HS Assay Kits, designed for use with Qubit Fluorometers, are highly selective for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA), RNA, protein, and free nucleotides. All kits provide a ready-to-use working solution and DNA standards. To perform the assay, simply dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a Qubit Fluorometer.

Qubit 1X dsDNA HS Assay Kit
The Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 5 pg/μL to 120 ng/μL, providing a detection range of 0.1−120 ng.

Notes
• Qubit 1X dsDNA HS can be used with Qubit 2, Qubit 3, Qubit 4, and Qubit Flex Fluorometers
• Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200 μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

Specifications

• Assay: 1X dsDNA, high sensitivity
• For Use With (Equipment): Qubit 2.0, 3, 4, and Flex Fluorometers
• No. of Reactions: 100 Reactions
• Quantitation Range: 0.1 to 120 ng
• Product Line: Qubit
• Quantity: 100 assays
• Shipping Condition: Wet Ice

Frequently Asked Questions (FAQs)

What is the expiration date for Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266) are stable for 6 months from the date of receipt when stored as directed.

For more information on product storage, please refer to the user guides:

Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit

What is the difference between The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) and Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854)?

The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) are newer and have a simplified workflow compared to the original Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854). The original Qubit kits contain separate dyes and buffer components that must be mixed together to make a working solution, in which the dye degrades after a few hours. With the newer Qubit 1X dsDNA Assay kits, the dye is premixed with a buffer that keeps the dye stable long-term and can be added directly to DNA samples.

You can find more information about the Qubit 1X dsDNA assay, by clicking on the Technical Note provided below:

Qubit 1X dsDNA assays: simplified workflow and improved performance

How should I store my Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. No. Q33230, Q33231, Q33265, Q33266)?

The Qubit 1X dsDNA High Sensitivity (HS) (Cat. Nos. Q33230, Q33231) and Broad Range (BR) Assay Kits (Cat. Nos. Q33265, Q33266) have different storage conditions.

For Qubit 1X dsDNA HS Assay Kits (Cat. Nos. Q33230, Q33231), store all components at 2 - 8 degrees C.

For Qubit 1X dsDNA BR Assay Kits (Cat. Nos. Q33265, Q33266), store the working solution at 18 - 28 degrees C. Store the standards at 2 - 8 degrees C.

The assay kits are stable for at least 6 months from the date of receipt.

For more information, please refer to these user guides: Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit