Invitrogen™ PureLink™ Microbiome DNA Purification Kit

Product Code. 15449733

Brand: Invitrogen™ A29790

Description

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications.

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility makes PureLink the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

• Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
• Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
• Streamlined protocols for numerous biological samples
• Recovery of highly pure DNA compatible with common downstream applications such as qPCR and next-generation sequencing

Certifications

Certificates of Analysis for each lot

Prestations de service

Product performance guaranteed for 1 year from date of sale

Spécification

• Column Type: Spin Column
• Content And Storage: • 40 mL S1 Lysis Buffer; room temperature
  • 5 mL S2 Lysis Enhancer; room temperature
  • 12.5 mL S3 Cleanup Buffer; room temperature
  • 45 mL S4 Binding Buffer; room temperature
  • 13 mL S5 Wash Buffer (concentrated); room temperature
  • 5 mL S6 Elution Buffer; room temperature
  • 50 PureLink Columns with Collection Tubes; room temperature
  • 100 PureLink Collection Tubes; room temperature
  • 50 Bead Tubes; room temperature
  
  
• Isolation Technology: Silica Spin Column
• Scale: Mini
• Sample Type: Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental
• Elution Volume: 50 to 200 μL
• Final Product Type: Microbial and host DNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps kit
• Shipping Condition: Room Temperature
• Test Time: Up to 55 min. (Including sample homogenization)
• Yield: ≤25 μg
• Starting Material Amount: Buccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab
  Microbial Culture: ≤5 mL
  Saliva: ≤2 mL
  Soil: ≤2 g
  Stool: ≤2 g
  Urine: ≤10 mL

Sécurité et traitement

Identifiant produit

• PureLink Microbiome DNA Purification Kit

Mention d'avertissement

• Warning

Catégorie de danger

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Mention de danger

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.

Conseils de prudence

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Informations supplémentaires

• MIXTURE LIST-Contains : Guanidine hydrochloride, Thiocyanic acid

For research use only. Not for use in diagnostic procedures.