Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit

Product Code. 12055825

Brand: Invitrogen™ 12173011A

Description

The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes.

The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design

The PureLink Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Specifications

• Content And Storage: • 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature
  • 250 mL PureLink Pro 96 Wash Buffer I; room temperature
  • 250 mL PureLink Pro 96 Wash Buffer II; room temperature
  • 75 mL PureLink Pro 96 RNase-free Water; room temperature
  • 4 PureLink 96-well Total RNA Filter Plates; room temperature
  • 4 PureLink Pro 96 Elution Plates; room temperature
• Isolation Technology: Silica Plate
• Purification Time: 25 min. (After sample homogenization)
• Elution Volume: 45–170 μL
• Sample Type: Bacteria, Cells, Plant, Tissue, Yeast
• Final Product Type: Total RNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Product Type: RNA Purification Kit
• Quantity: 384 Preps
• Scale: Mini
• Shipping Condition: Room Temperature
• Starting Material Amount: Bacteria: ≤10⁹ cells, Cells: varies, Plant: ≤150 mg, Tissue: ≤100 mg, Yeast: ≤1.8 mL
• Yield: Up to 33 μg

Safety and Handling

Product Identifier

• PureLink Pro 96 total RNA Purification Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P363-Wash contaminated clothing before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Protein denaturant,Thiocyanic acid

For Research Use Only. Not for use in diagnostic procedures.