PeproTech PSPN Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17824403	PeproTech 500-P138BT-25UG	25 μg
17884773	PeproTech 500-P138BT-50UG	50 μg
17814023	Invitrogen 500-P138BT-1MG	2 x 500 μg
30054658	PeproTech 500-P138BT-500UG	500 μg

Description

AA Sequence of recombinant protein: RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Persephin. Anti-Human Persephin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Persephin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Persephin (500-P138) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Persephin. Western Blot: To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPersephin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P138BT-1MG will be provided as 2 x 500 μg

Persephin is a disulfide-linked, homodimeric, neurotrophic factor structurally related to GDNF, artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken, and was later shown to be the preferential binding subunit for persephin. Persephin promotes the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve oxotomy, and, like GDNF, promotes ureteric bud branching. However, in contrast to GDNF and neurturin, persephin does not support the survival of peripheral neurons.

Specifications

• Antigen: PSPN
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: O60542
• Gene ID (Entrez): 5623
• Immunogen: E.coli-derived, 20.8 kDa Recombinant Human Persephin.
• Purification Method: Antigen affinity chromatography
• Gene: PSPN

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: Persephin; PSP; Pspn
• Gene Symbols: PSPN
• Primary or Secondary: Primary
• Content And Storage: -20°C