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  6. Macherey-Nagel™ Protein Quantification Assay

Macherey-Nagel™ Protein Quantification Assay

Product Code. 12779650
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Assay:
50
250
Unit Size:
250 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 12779650

Brand: Macherey-Nagel™ 740967.250

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This item is not returnable. View return policy

Description

Convenient and reliable kit for the determination of protein concentration.

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.

Superior selectivity - purify your RNA fractionated by size

  • Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)

Additional isolation of total protein

  • Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.

Excellent RNA recovery - even without phenol/chloroform

  • Lysis with chaotropic saltSpin column based procedure

Convenient handling - complete equipment provided

  • NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
  • Silica membrane technology
  • Format: minispin columns
  • Sample size: <107 cultured cells, <30mg human/tissue; <50 mg plant tissue, <150μL reaction mixture
  • Fragment size, small RNA: 18 to 200 bases
  • Fragment size, large RNA: >200 bases
  • Typical yield: 10μg small RNA; 95μg large RNA from 107 HeLa cells
  • Binding capacity: 200μg
  • Elution volume, 30μL to 100μL
  • Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
  • Small RNA (18 to 200 bases)
  • Large RNA (>200 bases)
  • Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.

Specifications

Concentration 10 to 20000ng/μL
Detectable Analytes Protein
Assay 250
Immunoassay Kit Format Buffer and Reagent Set
Product Type Protein Quantification Assay
Sample Type Protein
Sufficient For 107
For Use With (Application) Small and large RNA and total RNA purification
Kit Contents Chaotropic salt
Quantity 1 Each
Test Time 40 min.
Total Assay Time 40 min.
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Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Substances and mixtures corrosive to metals Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H290-May be corrosive to metals.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Supplemental information
  • MIXTURE LIST-Contains : hydrochloric acid
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