R&D Systems™ ProPak™ Recombinant Human IL-2 GMP Protein, CF

Product Code. 30713221

Brand: R&D Systems™ PPK002GMPIU3M

Description

Recombinant Protein

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The potency of recombinant human IL-2 is 75-125% of the IU pack size. International units are calibrated from an internal reference standard that is value assigned against the human IL-2 WHO International Standard (NIBSC code: 86/500).

Specifications

• Accession Number: P60568.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS, recombinant HSA, and Trehalose.
• Gene ID (Entrez): 3558
• Molecular Weight (g/mol): M.W.-observed: 13 kDa, under reducing conditions., M.W.-theroretical: 15.5 kDa
• Quantity: 3,000,000 IU
• Source: E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidlelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 6 months when stored between -14 °C and -40 °C. Can be stored up to 2 weeks at 2-8 °C. Refer to lot specific COA for the Use by Date.
• Endotoxin Concentration: <5.0 EU/mL by the LAL method.
• Gene Alias: aldesleukin, IL2, IL-2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, Proleukin, T cell growth factor, T-cell growth factor, TCGF
• Conjugate: Unconjugated
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 15521 ± 5 Da, and a second 15390 ± 5 Da product may be present.
• Protein: IL-2